Diagnosing Pancreatic Cancer Using Methylation Specific PCR Analysis
نویسندگان
چکیده
منابع مشابه
Methylation-specific PCR.
Methylation of the DNA is an important epigenetic (i.e., not associated with alteration in the primary structure of the DNA) phenomenon, which plays important roles in regulation of gene expression, maintenance of genome integrity, and genomic imprinting. Although not only other nucleotides, but also proteins and lipids can be methylated, in the context of the present discussion, “methylation” ...
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Methylation-specific PCR (MSP) is a simple, quick and cost-effective method to analyze the DNA methylation status of virtually any group of CpG sites within a CpG island. The technique comprises two parts: (1) sodium bisulfite conversion of unmethylated cytosine's to uracil under conditions whereby methylated cytosines remains unchanged and (2) detection of the bisulfite induced sequence differ...
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Gastric Cancer (GC) is the second most common cancer in the world and a leading cause of cancer-related mortality. Methylation of promoter CpG islands (CGIs) belonging to tumor suppressor genes causes transcriptional silencing of their corresponding genes leading to carcinogenesis and other disorders. Adenomatous Polyposis Coli (APC) a tumor suppressor gene is inactivated by methylation of prom...
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introduction: promoter methylation has an inhibitory effect on gene expression. thus we investigated methylation patterns of p15 gene in esophageal squamous cell carcinoma (escc), one of the most frequent and fetal cancers in the world. material and methods: p15 promoter methylation status of 30 escc and 10 normal specimens was investigated using methylation specific pcr. dna extracted from the...
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ژورنال
عنوان ژورنال: Cancer Biology & Therapy
سال: 2003
ISSN: 1538-4047,1555-8576
DOI: 10.4161/cbt.183